The Ents appear in The Lord of the Rings as ancient shepherds of the forest and allies of the free peoples of Middle-earth during the War of the Ring. The Ent who figures most prominently in the book is Treebeard, who (credibly) claims to be the oldest creature in Middle-earth. At the time The Lord of the Rings takes place, there are no young Ents (Entings) because the Entwives (female Ents) were lost. The Ents are akin to Huorns, whom Treebeard describes as a transitional form of trees which become animated or, conversely, as Ents who grow more "treelike" over time.
Inspired by Tolkien and similar traditions, animated or anthropomorphic tree creatures appear in a variety of media and works of fantasy.
The word "Ent" was taken from the Anglo-Saxon (Old English) word ent, meaning "giant". Tolkien borrowed the word from the Anglo-Saxon phrases orþanc enta geweorc ("work of cunning giants") and eald enta geweorc ("old work of giants", which describes Roman ruins). In this sense, Ents are probably the most ubiquitous of all creatures in fantasy and folklore, perhaps second only to dragons, for the word can refer to a variety of large, roughly humanoid creatures, such as giants, orcs, trolls, or even the monster Grendel from the poem Beowulf.
The plasma membrane monoamine transporter (PMAT) is a low-affinity monoamine transporterprotein which in humans is encoded by the SLC29A4gene. It is known alternatively as the human equilibrative nucleoside transporter-4 (hENT4). Unlike other members of the ENT family, it is impermeable to most nucleosides, with the exception of the inhibitory neurotransmitter and ribonucleosideadenosine, which it is permeable to in a highly pH-dependent manner.
This protein is an integral membrane protein that transports the monoamine neurotransmitters (serotonin, dopamine, norepinephrine) as well as adenosine, from synaptic spaces into presynaptic neurons or neighboring glial cells. It is abundantly expressed in the human brain, heart tissue, and skeletal muscle, as well as in the kidneys. It is relatively insensitive to the high affinity inhibitors (such as SSRIs) of the SLC6A monoamine transporters (SERT, DAT, NET), as well being only weakly sensitive to the adenosine transport inhibitor, dipyridamole. Its transport of monoamines, unlike for adenosine, is pH-insensitive. At low pH, (5.5-6.5 range, as occurs under ischemic conditions) however, its transport efficiency for adenosine becomes greater than for serotonin.
During 1977 and 1978, bassist Marino Pelajić, guitarist Mladen Jurčić, and drummer Branko Hromatko were Azra members when Branimir "Johnny" Štulić brought Jura Stublić as the new vocalist. Stublić was to become Aerodrom member, but due to his deep vocals it never happened. The lineup functioned for a few months only and after a quarrel with Štulić, on early 1979, Pelajić, Jurčić, Hromatko and Stublić formed the band Šporko Šalaporko i Negove Žaluzine, naming the band after a story from the "Polet" youth magazine, which was soon after renamed to Film. The memories of the Azra lineup later inspired Štulić to write the song "Roll over Jura" released on Filigranski pločnici in 1982.
Saxophonist Jurij Novoselić, who at the time had worked under the pseudonym Kuzma Videosex, joined the band, inspiring others to use pseudonym instead of their original names: vocalist Stublić became Jura Jupiter, bassist Pelajić became Mario Baraccuda and guitarist Jurčić became Max Wilson. Before joining the band, Stublić did not have much experience as a vocalist, however, since his father had been an opera singer, he often visited the theatre and opera, and at the age of 13, he started playing the guitar, earning money as a street performer at seaside resorts.
Beckett’s original choice for the lead – referred to only as “O” – was Charlie Chaplin, but his script never reached him. Both Beckett and the director Alan Schneider were interested in Zero Mostel and Jack MacGowran. However, the former was unavailable and the latter, who accepted at first, became unavailable due to his role in a "Hollywood epic." Beckett then suggested Buster Keaton. Schneider promptly flew to Los Angeles and persuaded Keaton to accept the role along with "a handsome fee for less than three weeks' work."James Karen, who was to have a small part in the film, also encouraged Schneider to contact Keaton.
The filmed version differs from Beckett's original script but with his approval since he was on set all the time, this being his only visit to the United States. The script printed in Collected Shorter Plays of Samuel Beckett (Faber and Faber, 1984) states: